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Cross-species testing of 27 pre-existing microsatellites in Podarcis gaigeae and Podarcis hispanica (Squamata: Lacertidae)

Author:
  • Anna Runemark
  • Marianne Gabirot
  • Staffan Bensch
  • Erik Svensson
  • Jose Martin
  • Panayiotis Pafilis
  • Efstratios D. Valakos
  • Bengt Hansson
Publishing year: 2008
Language: English
Pages: 1367-1370
Publication/Series: Molecular Ecology Resources
Volume: 8
Issue: 6
Document type: Journal article
Publisher: Wiley-Blackwell

Abstract english

We tested 27 microsatellite loci for cross-species amplification in the lacertids Podarcis gaigeae and Podarcis hispanica. We detected 11 and 15 polymorphic loci in the former and the latter species, respectively. In a larger sample of individuals from a single population of each species, the number of alleles ranged from five to 23 in 10 of the polymorphic loci in P. gaigeae, and between four and 13 in nine of polymorphic loci in P. hispanica. Two locus deviated from Hardy-Weinberg equilibrium in P. hispanica. Between 11 and 16 of the 27 loci also amplified successfully in three other Podarcis species.

Keywords

  • Biological Sciences
  • neutral molecular marker
  • cross-species amplification
  • microsatellite
  • Podarcis
  • population differentiation
  • Squamata

Other

Published
  • ISSN: 1755-098X
erik_svensson
E-mail: erik.svensson [at] biol.lu.se

Professor

Evolutionary ecology

+46 46 222 38 19

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Centre for Animal Movement Research
Evolutionary Ecology, Department of Biology
Ecology building S-223 62 Lund Sweden