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Ten polymorphic microsatellite markers for Hieracium s.s. (Asteraceae)

Author:
  • Jane Jönsson
  • Maren Wellenreuther
  • Torbjörn Tyler
Publishing year: 2010
Language: English
Pages: 295-300
Publication/Series: Conservation Genetics Resources
Volume: 2
Issue: 1
Document type: Journal article
Publisher: Springer

Abstract english

Eight novel microsatellite markers were developed for Hieracium s.s. (excluding Pilosella) using a modified FIASCO protocol, and two out of nine microsatellites that were originally developed for Pilosella officinarum were optimised for Hieracium s.s.. All ten microsatellites consistently amplified loci from a wide range of representatives from north and central European species of various sections of the genus. When tested on 19 individuals, nine microsatellites were found to be highly polymorphic with 6–29 alleles, while one microsatellite marker was monomorphic. Cross-amplification showed that six of the ten microsatellites also amplified products in one taxon of Pilosella, P. floribunda × officinarum.

Keywords

  • Biological Sciences
  • Botany
  • microsatellite marker
  • FIASCO
  • Hieracium
  • Pilosella
  • genetic variation
  • cross-amplification

Other

Published
  • Molecular Ecology and Evolution Lab
  • ISSN: 1877-7260
Jane Jönsson
E-mail: jane.jonsson [at] biol.lu.se

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