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Staining-free malaria diagnostics by multispectral and multimodality light-emitting-diode microscopy.

Author:
  • Aboma Merdasa
  • Mikkel Brydegaard
  • Sune Svanberg
  • Jeremie T Zoueu
Publishing year: 2013
Language: English
Publication/Series: Journal of Biomedical Optics
Volume: 18
Issue: 3
Document type: Journal article
Publisher: Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society

Abstract english

We report an accurate optical differentiation technique between healthy and malaria-infected erythrocytes by quasi-simultaneous measurements of transmittance, reflectance, and scattering properties of unstained blood smears using a multispectral and multimode light-emitting diode microscope. We propose a technique for automated imaging, identification, and counting of malaria-infected erythrocytes for real-time and cost-effective parasitaemia diagnosis as an effective alternative to the manual screening of stained blood smears, now considered to be the gold standard in malaria diagnosis. We evaluate the performance of our algorithm against manual estimations of an expert and show a spectrally resolved increased scattering from malaria-infected blood cells.

Keywords

  • Bioengineering Equipment

Other

Published
  • ISSN: 1083-3668
Mikkel Brygdegaard
E-mail: mikkel.brydegaard [at] forbrf.lth.se

Associate senior lecturer

Combustion Physics

+46 46 222 76 57

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Centre for Animal Movement Research
Evolutionary Ecology, Department of Biology
Ecology building S-223 62 Lund Sweden