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Differences in islet-enriched miRNAs in healthy and glucose intolerant human subjects.

Author:
  • Caroline Bolmeson
  • Jonathan Esguerra
  • S Albert Salehi
  • Dina Speidel
  • Lena Eliasson
  • Corrado Cilio
Publishing year: 2011
Language: English
Pages: 16-22
Publication/Series: Biochemical and Biophysical Research Communications
Volume: Dec
Document type: Journal article
Publisher: Elsevier

Abstract english

Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 were positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 were negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors (HbA1c⩾6.1). We suggest the presence of an islet-specific miRNA network, which consists of at least miR-375, miR-127-3p and miR-184, potentially involved in insulin secretion. Our results provide new insight into miRNA-mediated regulation of insulin secretion in healthy and glucose intolerant subjects.

Keywords

  • Biological Sciences

Other

Published
  • Islet cell exocytosis
  • Islet cell physiology
  • Cellular Autoimmunity
  • ISSN: 1090-2104
Caroline Bolmeson
E-mail: caroline [dot] bolmeson [at] biol [dot] lu [dot] se

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