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Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring

  • Haiyan Xie
  • Zhiyuan Xie
  • Monirehalsadat Mousavi
  • Niels Bendsöe
  • Mikkel Brydegaard
  • Johan Axelsson
  • Stefan Andersson-Engels
Publishing year: 2014
Language: English
Pages: 11-071408
Publication/Series: Journal of Biomedical Optics
Volume: 19
Issue: 7
Document type: Journal article
Publisher: Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society

Abstract english

Abstract in Undetermined

ABSTRACT. Optical techniques for tissue diagnostics currently are experiencing tremendous growth in biomedical applications, mainly due to their noninvasive, inexpensive, and real-time functionality. Here, we demonstrate a hand-held fiber optic probe instrument based on fluorescence/reflectance spectroscopy for precise tumor delineation. It is mainly aimed for brain tumor resection guidance with clinical adaptation to minimize the disruption of the standard surgical workflow and is meant as a complement to the state-of-the-art fluorescence surgical microscopy technique. Multiple light sources with fast pulse modulation and detection enable precise quantification of protoporphyrin IX (PpIX), tissue optical properties, and ambient light suppression. Laboratory measurements show the system is insensitive to strong ambient light. Validation measurements of tissue phantoms using nonlinear least squares support vector machines (LS-SVM) regression analysis demonstrate an error of <5% for PpIX concentration ranging from 400 to 1000 nM, even in the presence of large variations in phantom optical properties. The mean error is 3% for reduced scattering coefficient and 5% for blood concentration. Diagnostic precision of 100% was obtained by LS-SVM classification for in vivo skin tumors with topically applied 5-aminolevulinic acid during photodynamic therapy. The probe could easily be generalized to other tissue types and fluorophores for therapy guidance and monitoring.


  • Bioengineering Equipment
  • fluorescence spectroscopy
  • fiber optic sensors
  • reflectance
  • tissue
  • light-emitting diodes


  • Biophotonics-lup-obsolete
  • ISSN: 1083-3668
Mikkel Brygdegaard
E-mail: mikkel [dot] brydegaard [at] forbrf [dot] lth [dot] se

Associate senior lecturer

Combustion Physics

+46 46 222 76 57



Centre for Animal Movement Research
Evolutionary Ecology, Department of Biology
Ecology building S-223 62 Lund Sweden