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In vivo measurements of diffuse reflectance and time-resolved autofluorescence emission spectra of basal cell carcinomas

  • Alex J. Thompson
  • Sergio Coda
  • Mikkel Brydegaard
  • Gordon Kennedy
  • Rakesh Patalay
  • Ulrika Waitong-Bramming
  • Pieter A. A. De Beule
  • Mark A. A. Neil
  • Stefan Andersson-Engels
  • Niels Bendsöe
  • Paul M. W. French
  • Katarina Svanberg
  • Chris Dunsby
Publishing year: 2012
Language: English
Pages: 240-254
Publication/Series: Journal of Biophotonics
Volume: 5
Issue: 3
Document type: Journal article (comment)
Publisher: John Wiley & Sons

Abstract english

We present a clinical investigation of diffuse reflectance and time-resolved autofluorescence spectra of skin cancer with an emphasis on basal cell carcinoma. A total of 25 patients were measured using a compact steady-state diffuse reflectance/fluorescence spectrometer and a fibre-optic-coupled multispectral time-resolved spectrofluorometer. Measurements were performed in vivo prior to surgical excision of the investigated region. Singular value decomposition was used to reduce the dimensionality of steady state diffuse reflectance and fluorescence spectra. Linear discriminant analysis was then applied to the measurements of basal cell carcinomas (BCCs) and used to predict the tissue disease state with a leave-one-out methodology. This approach was able to correctly diagnose 87% of the BCCs. With 445 nm excitation a decrease in the spectrally averaged fluorescence lifetime was observed between normal tissue and BCC lesions with a mean value of 886 ps. Furthermore, the fluorescence lifetime for BCCs was lower than that of the surrounding healthy tissue in all cases and statistical analysis of the data revealed that this decrease was significant (p = 0.002). (C) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)


  • Dermatology and Venereal Diseases
  • Cancer and Oncology
  • fluorescence
  • fluorescence lifetime
  • diffuse reflectance
  • skin cancer
  • spectrometer
  • fibre probe


  • Biophotonics-lup-obsolete
  • ISSN: 1864-063X
Mikkel Brygdegaard
E-mail: mikkel [dot] brydegaard [at] forbrf [dot] lth [dot] se

Associate senior lecturer

Combustion Physics

+46 46 222 76 57



Centre for Animal Movement Research
Evolutionary Ecology, Department of Biology
Ecology building S-223 62 Lund Sweden